TELL-Seq™ Kit from Universal Sequencing Technology enables the construction of NGS libraries with 200Kb fragments that can be sequenced with Illumina instruments. This transposase-based technology has a highly simplified, scalable protocol that takes 3 hours, can be performed in a PCR tube, and requires minimal DNA (0.2 ng for bacteria and 3 ng for humans). It’s an excellent solution for Whole Genome sequencing, Whole Genome phasing, de-novo assembly, metagenomics, and structural variant analysis.
These kits allow the preparation of RNA-Seq libraries that include UMI and UDI for whole transcriptome analysis using Illumina NGS platforms. The established CORALL technology, free from mechanical or enzymatic fragmentation, is completed in 6 steps (4.5 hours). It can be applied to all sample types, including degraded and FFPE samples, and offers exceptional performance on low-input samples, down to 1 ng of initial quantity before mRNA selection or ribo-depletion.
Library preparation can be done starting directly from total RNA or enriched small RNA. The protocol takes 5 hours and the resulting libraries do not require gel purification. It is highly sensitive and therefore suitable for samples with low RNA content, exosomes, and liquid biopsies. The required sample range is from 100ng to 1,000ng of total RNA or from 50pg-1,000ng of enriched small RNA, including plasma, serum, and urine. It can include the TraPR kit for purification of only physiologically active Small RNAs.
QuantSeq is the “gold standard” solution for differential expression analysis that allows for cost containment in sequencing while maintaining result quality. This approach creates a single fragment at the 3′ UTR of each messenger with a protocol that doesn’t require poly(A) enrichment or rRNA depletion. The procedure is completed in 4.5 hours and can be successfully applied to degraded samples (e.g., FFPE) and blood samples where, thanks to the Globin Block Module, fragments from globin mRNA are not generated. The protocol is scalable and automatable, and in the REV version, it allows for the study of alternative polyadenylation sites.
Traditional single-cell RNA-Seq methods are based on sequencing thousands of cells with low read depth. While this approach is sufficient to identify cell types based on the detection of highly expressed genes, it provides an incomplete picture of expressed genes and doesn’t allow for fine characterization of cell subpopulations. LUTHOR HD, thanks to direct RNA “amplification, e” nables high-definition scRNA-Seq with unprecedented sensitivity and resolution, allowing the capture and sequencing of even poorly expressed genes and revealing the complete and real transcriptome of each cell (typically 95% of expressed genes with a read depth of 1M). It provides the best results even with very low sample amounts: 1-100 cells or 10 pg-1 ng of total RNA, with the possibility of going down to 0.15 pg. It doesn’t require RNA extraction, mRNA enrichment, or RNA depletion. Additionally, the “elimination of gDNA reduces bias, simplifies the” data analysis, and allows for cost savings. The protocol takes 6 hours and can be applied to single eukaryotic cells, cell biopsies, or purified total RNA
The tagmentation protocol, which simultaneously fragments and ligates adapters for NGS DNA library preparation, has been perfected by SeqWell in a way never achieved before. This was possible by optimizing each kit reagent, but especially by engineering the Tn5 Transposase into a patented form called TnX that guarantees absolute reliability and specificity of operation. The library preparation protocol is thus reduced to just 3 steps (reaction setup – libraries amplification – pooling and purification) for 100 minutes, allowing up to 96 samples to be prepared simultaneously and therefore enabling extremely high throughput.
3D genomics provides unprecedented access to the sequence, structure, and regulatory landscape of any genome. The Hi-C (High Throughput Chromatin Conformation Capture) technique that allows visualization of how temporal and spatial changes in chromatin alter gene regulation and cellular functions, combines the Proximity Ligation assay with the NGS approach.
This technique has been optimized in Arima Hi-C kits to achieve a more flexible, robust, and faster protocol that can be done in a single tube using 2 or 4 patented restriction enzymes and producing a much higher percentage of long-range cis interactions compared to the classic approach at a lower cost. There are multiple applications for 3D genomics. In cancer research, it enables the discovery of structural variants for a complete understanding of disease mechanisms, biomarker discovery, and new therapeutic approaches thanks to insights into the sequence, structure, and regulatory landscape of cancer genomes.
Understanding how spatial relationships in genome structure influence gene regulation, cell development, and pathological processes supports epigenetic studies, while the “assembly of chromosome-scale genomes helps explore the” evolution of species.
