3D genomics provides unprecedented access to the sequence, structure, and regulatory landscape of any genome. The Hi-C (High Throughput Chromatin Conformation Capture) technique that allows visualization of how temporal and spatial changes in chromatin alter gene regulation and cellular functions, combines the Proximity Ligation assay with the NGS approach.
This technique has been optimized in Arima Hi-C kits to achieve a more flexible, robust, and faster protocol that can be done in a single tube using 2 or 4 patented restriction enzymes and producing a much higher percentage of long-range cis interactions compared to the classic approach at a lower cost. There are multiple applications for 3D genomics. In cancer research, it enables the discovery of structural variants for a complete understanding of disease mechanisms, biomarker discovery, and new therapeutic approaches thanks to insights into the sequence, structure, and regulatory landscape of cancer genomes.
Understanding how spatial relationships in genome structure influence gene regulation, cell development, and pathological processes supports epigenetic studies, while the “assembly of chromosome-scale genomes helps explore the” evolution of species.
