Flow Cytometry Line

A broad and complete line for flow cytometric analysis.

Prodotti Gianni collaborates with various partners to provide researchers with a broad and complete line for flow cytometric analysis.

Instruments
Reagents
Antibody conjugation kits
Solutions for exosome analysis
Reagents for multiplexing analysis

Instruments

Cytek flow cytometers with unique patented technologies for rapid and high-precision analysis.

Guava MUSE: Compact benchtop instrument, easy to use and can be placed anywhere in the lab, including inside a sterile hood.

Touchscreen graphical interface with intuitive acquisition software.
Patented capillary flow cell, requires no sheath fluid and produces minimal waste volumes.
Works with pre-optimized kits for: cell counting and viability, cell health assays (AnnV, Caspase, Mitochondrial Potential, …), cell cycle analysis, cell signaling, immunology (CD4 and CD8).

Guava EASYCYTE: Works with pre-optimized kits for various cellular activities and, in an open manner, with fluorescent markers.

Single tube sample loader (SL) or with 96-well plate sampler (HT).
Configurable from one to 3 lasers, up to 14 total parameters. Upgrade possible.
Functions using capillary fluidics, requires no sheath fluid.
Main applications: Multiparametric immunophenotyping (immunology, cancer research), Cell counting and viability (drug toxin screening), Exotic Flow Cytometry (microbiology, algae, seminal fluid, seeds, …), Bacteria, Gram typing.

Amnis ImageStream: Visual flow cytometer that allows acquisition and saving of images with 20x, 40x and 60x magnification. Signal detection via CCD camera managed in time delay integration (TDI) mode.

High-sensitivity instrument, characterized by multiple functions such as AI and Machine Learning capable of enhancing any flow cytometry application.

Reagents

Antibodies conjugated with classic fluorochromes and brilliant new generation dyes.
Isotype controls
Compensation beads
Reagents and kits for cell viability and apoptosis studies: Annexin V, Tunel kit, Mitochondrial Membrane Potential Assay, Caspase Analysis, Live/Dead Reagents, Reagents for intracellular staining, Sars-CoV2 Reagents

Conjugation Kits

Abcam’s innovative Lightning-Link® technology enables direct conjugation of antibodies or proteins with HRP, FITC, PE, biotin and other enzymes, fluorophores and small molecules.

Quick and extremely simple conjugation system.
Minimal amounts of starting material required.
Various formats available and customization possibilities

Exosome Analysis

Reagents and complete kits for simple and precise detection and characterization of Exosomes.

Single antibodies for recognition of specific target molecules.
Panel of specific antibodies for exosome recognition.
Capture and enrichment beads for exosomes (CD9, CD63, CD81, CD274, CD326).
Lyophilized exosome standards to use as controls.
Kits for isolation with capture beads and immunophenotyping of exosomes with sample-specific markers. Simultaneous isolation and immunophenotyping (CD9+CD63 for cell cultures and urine, CD63+CD81 for plasma).

Multiplexing Analysis

Quantitative analysis of up to 25 analytes simultaneously using flow cytometry

Cytometric Bead Array technology for high-throughput protein detection using a standard flow cytometer.
Sandwich immunoassay principle, where a target protein is immobilized between a capture antibody locked on magnetic microspheres and a detection antibody conjugated with a fluorophore.
Simultaneous analysis of multiple target proteins using different combinations of microsphere sizes and fluorophores.
Compatible with most standard flow cytometers equipped with blue and red lasers capable of detecting fluorescence in the phycoerythrin (PE) and allophycocyanin (APC) channels, respectively.
Up to 25 different proteins can be analyzed with just 25μl of sample.
Rapid technique that is more economical than classic ELISA or WB assays.
Customizable.

This technology enables the design and creation of assays to measure a variety of analytes including inflammatory mediators, chemokines, immunoglobulin isotypes, intracellular signaling molecules, apoptotic mediators, adhesion molecules, and antibodies.

Main Applications:

Simultaneous analysis of various cytokines (e.g., inflammatory cytokine analysis).
Identification of potential molecular targets for drug development.
Identification of molecular mechanisms of drug action.
Research for new biomarkers.
Identification of various factors involved in disease expression processes.

Life science