Gianni Products Service

Prodotti Gianni provides the “extensive experience of its partners for the” reliable execution of service experiments and custom production requests.

Next Generation Sequencing

Whole genome sequencing: identification of SNPs, CNVs, PAVs, insertions, deletions, translocations
Bisulfite treatment: evaluation of DNA methylation levels
Whole exome sequencing: sequencing of only the coding part with the possibility of selecting exons of interest
Amplicon sequencing: clinical genotyping projects for analyzing numerous samples at reduced costs
Long reads sequencing: for the reconstruction of complex genomes
Metagenomics: profiling of the taxonomic composition of complex biological substrates
Hi-C Seq: study of chromatin interactions in 3D space by linking chromosomal structure directly to genomic sequence.
Total RNAseq: quantification of total RNA
mRNAseq: quantification through complete sequencing of all messenger RNAs
Small RNAseq: quantification of Small RNAs only. Possibility to select only physiologically relevant Small RNAs
QuantSeq: differential expression analysis. Gold standard for particularly challenging samples such as FFPE or degraded samples

SINGLE Cell RNAseq in HD version
Up to 15,000 genes. Quantification of “RNA from A SINGLE nucleus or A SINGLE CYTOPLASM thanks to the patented approach of” direct “amplification of” RNA (minimum RNA input – 0.15pg)
3D Genomics
Analysis of the three-dimensional organization of DNA in the nucleus for a better understanding of gene regulation, cell development, disease mechanisms and progression, biomarker research, and genome assembly
Spatial transcriptomics
Single Cell transcriptomic analysis on histological samples using NGS Stereo-Seq approach.

Gene synthesis (up to 40,000 bases) with “codon optimization”
Oligo and peptide synthesis
Cloning and subcloning
Mutagenesis
RNA synthesis
DNA library synthesis (site-directed mutagenesis library, random mutant library, degenerated library, controlled library, sgRNA library)
CRISPR/Cas9

Up to 45 cytokines quantified simultaneously in 25μl of serum/plasma/cell supernatant
Negative control consisting of cytokine-depleted serum for exact quantification
Significant reduction in costs and time compared to the corresponding ELISA approach

Quantification of over 1,000 proteins simultaneously in serum/plasma/cell supernatant samples
Quantification of glycosylation and phosphorylation levels

cDNA cloning
Expression in 5 cell lines (E.Coli, yeast, insect cells, CHO, HEK293)
Availability of different tags (HA, GST, His, hFc, AVI, no tag)
Possibility of bulk production
Monoclonal and polyclonal antibodies
Bifunctional antibodies
Humanized antibodies
Nanobodies (antibodies with MW 12-15 KDa)
Conjugation of antibodies/nanobodies
Coating of beads with antibodies/proteins
Development of ELISA kits: antibody labeling, antibody pair screening, assay development and optimization, production
Production of proteins/antibodies with patented “cell free” system
Production of transmembrane proteins

Isolation and purification from any type of sample. Guarantee of optimal purity and recovery, compatible with any downstream processing or analysis
Characterization with different techniques (TAM, WB, NTA, FC, ELISA)
Conjugation to antibodies, nanobodies, agonist molecules, cytokines, anti-phagocytic molecules, reporters, fluorescent probes
Loading with therapeutic agents, RNA, DNA, peptides, ASOs, plasmids
Analysis of transcriptome, proteome, and phosphoproteome using cutting-edge mass spectrometry techniques

Custom production of APIs, production intermediates, building blocks, biochemical compounds
Setup of libraries selecting from > 20,000 chemical compounds of different nature (inhibitors, activators, natural products, peptides, antibodies) and choosing quantity, format (solid or in
DMSO), plate positioning and concentration.
CADD (computer-aided drug design) and virtual screening for the computational identification of structures most likely to bind a molecular target of interest.

Separation of protein samples by SDS-PAGE
Protein identification by LC-MS/MS
Differential proteomics experiments (“untargeted label free” type) associated with analysis
LC-MS/MS for identification and relative quantification of proteins (including statistical analysis)
“Targeted” analysis for relative and/or absolute quantification of proteins by MRM

Analysis of interactions between biomolecules or biomolecules and generic ligands through Surface Plasmon Resonance
Protein purification through affinity chromatography experiments and/or immunoprecipitation (Interactomics)